Following python source code counts the total number of contigs present in a file and extracts number of genes, Complementary DNA strand, and other information. This program will run on files downloaded from NCBI.
#Nadia Baig
fr=open("C:\\Users\\XYZ\\Desktop\\chromosome.gbk,r") #Path and file name
a=fr.read()
b=a.split("//\n") #split the whole file on //
length=len(b)#counting length
i=0
genes=[]
for base in b:
a=base.split("\n")
for bb in a:
if bb.startswith(" gene ") :
i=i+1
genes.append(i)
print(genes) # printing information about contigs having genes
total=0
j=0
while j<len(genes):
if genes[j]==0:
total=total+1 #counting contigs having no genes
j=j+1
Tgene=sum(genes)
print("total number of genes present in file",Tgene) #total number of genes present in contig
print("total number of contigs having no genes are",total)
# printing those genes which have an attached cds or mRNA
#Having attached CDS
cds=[]
i=0
for CDS in b:
c=CDS.split("\n")
for cds1 in c:
if cds1.startswith(" CDS " ):
i=i+1
cds.append(i)
i=0
print("details of CDS")
print(cds) #showing list of genes having attached cds
nad=sum(cds) #TOtal number of cds
print('total number of attached cds',nad)
#genes having attached mRNA
mrna=[]
i=0
for CDS in b:
c=CDS.split("\n")
for cds1 in c:
if cds1.startswith(" mRNA "):
i=i+1
mrna.append(i)
i=0
print("details of genes having attached mRNA")
print(mrna) # list of total number of attached mrna,s in each contig
msum=sum(mrna) #Total number of attached mrna,s in all contigs
print('total number of attached mrnas',msum)
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